procedure:)

We planned to exposed the water to the sun at time intervals of 2, 4, 6and 8 hours but some unexpected problems arose and we only managed to get the samples for 4, 4 &1/2 and 5 hours instead, which we thought would be indicative enough.

MATERIALS

-       Water from the reservoir

-       Measuring cups

-       Marker

-       Filter

-      zip blog bags

-      8 Petri dish

-       Agar

-       8 spreaders

-       Timer

Nutrient Agar

PROCEDURE

1.    Collect 5000of water from the reservoir.

2.    Filter 4000 water.

3.    Pour 500ml of water into 4 zip block bags respectively.

4.    Label (1), (2),  (3) and (4)on each of the bags using the marker.

5.    Place (1), (2) and (3) bags in a sunny place and (4) in a place without sunlight at all

6.   4 hours later, remove bag (1)

7.   4 & 1/2 hours later, remove bag (2)

8.   5 hours later, remove bag (3)

9.    Take spreaders, swipe the water from 1, 2 , 3 and 4 filtered water and wipe it on the agar in a zig-zag motion.

10. place the petri dishes at a temperature of 37 degrees for a day before taking it out of the controlled environment

11. Approximate the percentage of area the bacteria took up.

12. Repeat step 3-11 with another set of experiment